This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. The key features are: includes a glossary of over 200 terms important for understanding molecular biology; uses an inexpensive source of eukaryotic cells - great for schools on a budget; and, includes Methods Locator that provides instant access to the latest methods. It also contains clearly written, easy-to-follow, student-tested instructions: Sterile techniques; Phage titration; Gel electrophoresis of DNA; Restriction enzyme digestion; Plasmid isolation; Transformation of E Coli; Recombinant DNA cloning; Nick translation labeling; Nonradioactive primer labelling; Nonradioactive DNA detection; Southern blotting; Colony hybridization; Purification of plant DNA; RNA purification; Northern blotting; Purification of poly A+ RNA; and, Polymerase chain reaction (PCR).
- Limba : Engleza
- Cuprins : Transposon Mutagenesis of Escherichia coli. Recombinant DNA and Cloning. Southern Blot Analysis. Plant Genomic Southern Blotting with Probes for Low- and High-Copy-Number Genes. RNA Purification and Northern Blot Analysis. Polymerase Chain Reaction. Appendices. Glossary. Subject Index.
- Data Publicarii : 28 Nov 1995
- Format : Paperback
- Numar pagini : 280
- ISBN : 9780123977205