This work covers the most recent development of enzymatic organic synthesis, with particular focus on the use of isolated enzymes. It is organized into one introductory chapter dealing with the characteristics of enzymes as catalysts, and five chapters dealing with different types of chemical transformations. Methods for enzyme immobilization and stabilization, the use of enzymes in extreme environments, and the alteration of enzyme properties by chemical modification and site-directed mutagenesis for synthetic purposes are covered.
- Limba : Engleza
- Cuprins : Preface. Acknowledgements. General Aspects. Rate acceleration in enzyme-catalyzed reactions. Michaelis-Menten kinetics. Enzyme inhibition. Specificity. Improvement or alteration of enzyme specifity. Enzyme stabilization and reactor configuration. Cofactor regeneration. Enzyme catalysis in organic solvents. Multienzyme systems and metabolic engineering. Rational design of new enzymatic catalysts. Conclusion. References. Use of Hydrolytic Enzymes: Amidases, Proteases, Esterases, Lipases, Nitrilases, Phosphatases, Epoxide Hydrolases. Amidases. Protease-catalyzed peptide synthesis. Proteases that act as esterases. Acetylcholine esterase. Pig liver esterase. Phospholipases. Cholesterol esterase. Lipases. Nitrile hydrolysis enzymes. Epoxide hydrolase. Phosphatase. References. Oxidoreductions. Nicotinamide cofactor dependent oxidoreductions. Dehydrogenases which utilize ketoacids as substrates. Other NAD(P)-dependent dehydrogenases. Oxidoreductases that are metalloenzymes. References. C-C Bond Formation. Aldol conde
- Data Publicarii : 17 Jun 1994
- Format : Hardback
- Numar pagini : 392
- ISBN : 9780080359410