Written by experts in the field, Handbook of Endocrine Research Techniques is currently the only single source of up-to-date methods and strategies particularly useful in endocrinological research. As a resource for both the novice and experienced investigator, the book includes chapters which provide an introduction to the area, general concepts, detailed protocols, and extensive references.
- Limba : Engleza
- Cuprins : Hormone Assays. Radioimmunoassay (RIA): Concept and Types. Immunoradiometric (IRMA) Assays. Enzyme-Linked Immunoabsorbent Assays (ELISA). Development and Characterization of Polyclonal and Monoclonal Antibodies. Isolation of Polypeptide Hormones. Reverse Hemolytic Plaque Assays. Capillary Electrophoresis Coupled to Fluorescence Detection for the Determination of Multiple Neuropeptides. Immunoreactive Heterogeneity in Peptide Measurement. Why Use a Flow Cytometer for Endocrine Cell Analysis? Identification and Characterization of Insulin-Like Growth Factor Binding Proteins. Histochemical and in Situ Approaches. Localization of Peptides. Electron Microscopic Immunocytochemical Approaches to the Localization of Ligands, Receptors, Transducers, And Transporters. In Situ Hybridization for Hormone and Receptor Expression. Techniques for Receptors and Signal Transductors. Plasma Membrane Isolation Strategies for Cell Surface Receptors. Tyrosine Kinase Receptors. Involvement of Protein Tyrosine Phosphatase Activity i
- Data Publicarii : 15 Dec 1993
- Format : Hardback
- Numar pagini : 599
- ISBN : 9780122099205